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KMID : 1149220170250020155
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Korean Journal of Oriental Medical Prescription
2017 Volume.25 No. 2 p.155 ~ p.166
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Effect of Polygoni Multiflori Ramulus extract against arachidonic acid and iron-induced oxidative stress in HepG2 cell and CCl4-induced liver injury in mice
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Jeon Chang-Gwon
Jung Ji-Yun
Park Chung-A
Jee Seon-Young
Kim Sang-Chan
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Abstract
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Objectives : Polygoni Multiflori Ramulus has been widely used as a traditional medicinal herb for the treatment of insomnia, limb pain and itch. The extract of Polygoni Multiflori Ramulus (PMRE) is known to have a modulatory effect of many inflammatory responses. This study was performed to investigate the hepatoprotective effect of PMRE against arachidonic acid (AA) + iron-induced oxidative stress on HepG2 cell and carbon tetrachloride (CCl4)-induced liver injury on mice.
Methods : The effects of PMRE on cell viability was assessed by MTT assay. And flow cytometric analysis was performed to estimate the effects on mitochondrial function. To investigate its underlying mechanism, apoptosis-related proteins were analysed by using immunoblot analysis. In addition, ICR mouse were administrated (po) with the PMRE (30, 100 mg/kg) for 3 days and then, injected (ip) with CCl4 (0.5 ml/kg body weight) to induce acute liver damage. The level of pro-caspase-3 was measured.
Results : Treatment of PMRE increased relative cell viability, prevented a cleavage of poly (ADP ribose) polymerase and pro-caspase-3, and also reduced mitochondrial membrane permeability against AA + iron-induced oxidative stress. In addition, PMRE treatment decreased liver injury induced by CCl4, as evidenced by increases in pro-caspase-3 level.
Conclusions : These results demonstrate that PMRE has an ability to anti-oxidant and hepatoprotective effect against AA + iron-induced oxidative stress and CCl4-induced liver injury.
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KEYWORD
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Polygoni Multiflori Ramulus , Oxidative stress , Hepatoprotective effect
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